Translational_Unit

Part:BBa_K2243002:Design

Designed by: Chen Hong   Group: iGEM17_Peking   (2017-10-23)


D4_Bxb1 gp35


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 192
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal XhoI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1105
    Illegal NgoMIV site found at 1192
    Illegal AgeI site found at 242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1300

Usage

D4_Bxb1 gp35 is one of our best expression system. We added RBS D4 upstream the Bxb1 gp35 coding sequence (BBa_K22430012).

Biology

The Bxb1 coding sequence is the part BBa_K22430012.

Design Notes

We construct this structure by Gibson Assembly. The D4 RBS is generate by random mutation.


Source

Mycobacterium Phage Bxb1

References